pdgfrb antibodies Search Results


95
Miltenyi Biotec cd140b pe apb5 130 118 457 miltenyi biotec
Flow cytometry antibody panel used for characterization.
Cd140b Pe Apb5 130 118 457 Miltenyi Biotec, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Sino Biological human pdgfr𝛽 antibody
Flow cytometry antibody panel used for characterization.
Human Pdgfr𝛽 Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Proteintech a sma
Flow cytometry antibody panel used for characterization.
A Sma, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Aviva Systems phospho pdgfrb
Figure 4: Analyses of fusion gene <t>CD53-PDGFRB.</t> (A) RT-PCR analysis of CD53, PDGFRB and CD53-PDGFRB fusion transcripts in cell lines DERL-2 and DERL-7. NTC: no template control. (B) RQ-PCR analysis of CD53 in selected cell lines and primary cells. (C) RQ-PCR analysis of PDGFRB in selected cell lines and primary cells (left). Western blot analysis of PDGFRB (middle) and phospho- PDGFRB (right) was performed in selected cell lines. TUBA served as loading control. (D) RQ-PCR analysis of PDGFD in selected cell lines and primary cells (left). Data for PDGFD protein levels in the supernatants as obtained by ELISA are indicated (right). (E) RQ-PCR analysis for PDGFRB <t>and</t> <t>MSX1</t> of DERL-2 cells (left) and DERL-7 cells (right) treated for siRNA-mediated knockdown of PDGFRB. (F) RQ-PCR analysis of MSX1 in DERL-2 cells and DERL-7 cells treated with 1 ng/ml and 10 ng/ml recombinant PDGFD (left). RQ- PCR analysis for PDGFD and MSX1 of DERL-7 cells treated for siRNA-mediated knockdown of PDGFD (middle). RQ-PCR analysis for MSX1 of DERL-2 and DERL-7 cells treated with PDGFR kinase-inhibitor Dasatinib (right). (G) RQ-PCR analysis for PDGFRB and AUTS2 of DERL-2 cells (above) and DERL-7 cells (below) treated for siRNA-mediated knockdown of PDGFRB.
Phospho Pdgfrb, supplied by Aviva Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miltenyi Biotec cd140b miltenyi biotec human igg1 apc
Figure 4: Analyses of fusion gene <t>CD53-PDGFRB.</t> (A) RT-PCR analysis of CD53, PDGFRB and CD53-PDGFRB fusion transcripts in cell lines DERL-2 and DERL-7. NTC: no template control. (B) RQ-PCR analysis of CD53 in selected cell lines and primary cells. (C) RQ-PCR analysis of PDGFRB in selected cell lines and primary cells (left). Western blot analysis of PDGFRB (middle) and phospho- PDGFRB (right) was performed in selected cell lines. TUBA served as loading control. (D) RQ-PCR analysis of PDGFD in selected cell lines and primary cells (left). Data for PDGFD protein levels in the supernatants as obtained by ELISA are indicated (right). (E) RQ-PCR analysis for PDGFRB <t>and</t> <t>MSX1</t> of DERL-2 cells (left) and DERL-7 cells (right) treated for siRNA-mediated knockdown of PDGFRB. (F) RQ-PCR analysis of MSX1 in DERL-2 cells and DERL-7 cells treated with 1 ng/ml and 10 ng/ml recombinant PDGFD (left). RQ- PCR analysis for PDGFD and MSX1 of DERL-7 cells treated for siRNA-mediated knockdown of PDGFD (middle). RQ-PCR analysis for MSX1 of DERL-2 and DERL-7 cells treated with PDGFR kinase-inhibitor Dasatinib (right). (G) RQ-PCR analysis for PDGFRB and AUTS2 of DERL-2 cells (above) and DERL-7 cells (below) treated for siRNA-mediated knockdown of PDGFRB.
Cd140b Miltenyi Biotec Human Igg1 Apc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miltenyi Biotec cd140b apc antibody
Figure 4: Analyses of fusion gene <t>CD53-PDGFRB.</t> (A) RT-PCR analysis of CD53, PDGFRB and CD53-PDGFRB fusion transcripts in cell lines DERL-2 and DERL-7. NTC: no template control. (B) RQ-PCR analysis of CD53 in selected cell lines and primary cells. (C) RQ-PCR analysis of PDGFRB in selected cell lines and primary cells (left). Western blot analysis of PDGFRB (middle) and phospho- PDGFRB (right) was performed in selected cell lines. TUBA served as loading control. (D) RQ-PCR analysis of PDGFD in selected cell lines and primary cells (left). Data for PDGFD protein levels in the supernatants as obtained by ELISA are indicated (right). (E) RQ-PCR analysis for PDGFRB <t>and</t> <t>MSX1</t> of DERL-2 cells (left) and DERL-7 cells (right) treated for siRNA-mediated knockdown of PDGFRB. (F) RQ-PCR analysis of MSX1 in DERL-2 cells and DERL-7 cells treated with 1 ng/ml and 10 ng/ml recombinant PDGFD (left). RQ- PCR analysis for PDGFD and MSX1 of DERL-7 cells treated for siRNA-mediated knockdown of PDGFD (middle). RQ-PCR analysis for MSX1 of DERL-2 and DERL-7 cells treated with PDGFR kinase-inhibitor Dasatinib (right). (G) RQ-PCR analysis for PDGFRB and AUTS2 of DERL-2 cells (above) and DERL-7 cells (below) treated for siRNA-mediated knockdown of PDGFRB.
Cd140b Apc Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miltenyi Biotec cd140b pdgfrb pe conjugated antibodies
Figure 4: Analyses of fusion gene <t>CD53-PDGFRB.</t> (A) RT-PCR analysis of CD53, PDGFRB and CD53-PDGFRB fusion transcripts in cell lines DERL-2 and DERL-7. NTC: no template control. (B) RQ-PCR analysis of CD53 in selected cell lines and primary cells. (C) RQ-PCR analysis of PDGFRB in selected cell lines and primary cells (left). Western blot analysis of PDGFRB (middle) and phospho- PDGFRB (right) was performed in selected cell lines. TUBA served as loading control. (D) RQ-PCR analysis of PDGFD in selected cell lines and primary cells (left). Data for PDGFD protein levels in the supernatants as obtained by ELISA are indicated (right). (E) RQ-PCR analysis for PDGFRB <t>and</t> <t>MSX1</t> of DERL-2 cells (left) and DERL-7 cells (right) treated for siRNA-mediated knockdown of PDGFRB. (F) RQ-PCR analysis of MSX1 in DERL-2 cells and DERL-7 cells treated with 1 ng/ml and 10 ng/ml recombinant PDGFD (left). RQ- PCR analysis for PDGFD and MSX1 of DERL-7 cells treated for siRNA-mediated knockdown of PDGFD (middle). RQ-PCR analysis for MSX1 of DERL-2 and DERL-7 cells treated with PDGFR kinase-inhibitor Dasatinib (right). (G) RQ-PCR analysis for PDGFRB and AUTS2 of DERL-2 cells (above) and DERL-7 cells (below) treated for siRNA-mediated knockdown of PDGFRB.
Cd140b Pdgfrb Pe Conjugated Antibodies, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miltenyi Biotec cd140b
Figure 4: Analyses of fusion gene <t>CD53-PDGFRB.</t> (A) RT-PCR analysis of CD53, PDGFRB and CD53-PDGFRB fusion transcripts in cell lines DERL-2 and DERL-7. NTC: no template control. (B) RQ-PCR analysis of CD53 in selected cell lines and primary cells. (C) RQ-PCR analysis of PDGFRB in selected cell lines and primary cells (left). Western blot analysis of PDGFRB (middle) and phospho- PDGFRB (right) was performed in selected cell lines. TUBA served as loading control. (D) RQ-PCR analysis of PDGFD in selected cell lines and primary cells (left). Data for PDGFD protein levels in the supernatants as obtained by ELISA are indicated (right). (E) RQ-PCR analysis for PDGFRB <t>and</t> <t>MSX1</t> of DERL-2 cells (left) and DERL-7 cells (right) treated for siRNA-mediated knockdown of PDGFRB. (F) RQ-PCR analysis of MSX1 in DERL-2 cells and DERL-7 cells treated with 1 ng/ml and 10 ng/ml recombinant PDGFD (left). RQ- PCR analysis for PDGFD and MSX1 of DERL-7 cells treated for siRNA-mediated knockdown of PDGFD (middle). RQ-PCR analysis for MSX1 of DERL-2 and DERL-7 cells treated with PDGFR kinase-inhibitor Dasatinib (right). (G) RQ-PCR analysis for PDGFRB and AUTS2 of DERL-2 cells (above) and DERL-7 cells (below) treated for siRNA-mediated knockdown of PDGFRB.
Cd140b, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
St Johns Laboratory rabbit anti pdgfr β
Figure 4: Analyses of fusion gene <t>CD53-PDGFRB.</t> (A) RT-PCR analysis of CD53, PDGFRB and CD53-PDGFRB fusion transcripts in cell lines DERL-2 and DERL-7. NTC: no template control. (B) RQ-PCR analysis of CD53 in selected cell lines and primary cells. (C) RQ-PCR analysis of PDGFRB in selected cell lines and primary cells (left). Western blot analysis of PDGFRB (middle) and phospho- PDGFRB (right) was performed in selected cell lines. TUBA served as loading control. (D) RQ-PCR analysis of PDGFD in selected cell lines and primary cells (left). Data for PDGFD protein levels in the supernatants as obtained by ELISA are indicated (right). (E) RQ-PCR analysis for PDGFRB <t>and</t> <t>MSX1</t> of DERL-2 cells (left) and DERL-7 cells (right) treated for siRNA-mediated knockdown of PDGFRB. (F) RQ-PCR analysis of MSX1 in DERL-2 cells and DERL-7 cells treated with 1 ng/ml and 10 ng/ml recombinant PDGFD (left). RQ- PCR analysis for PDGFD and MSX1 of DERL-7 cells treated for siRNA-mediated knockdown of PDGFD (middle). RQ-PCR analysis for MSX1 of DERL-2 and DERL-7 cells treated with PDGFR kinase-inhibitor Dasatinib (right). (G) RQ-PCR analysis for PDGFRB and AUTS2 of DERL-2 cells (above) and DERL-7 cells (below) treated for siRNA-mediated knockdown of PDGFRB.
Rabbit Anti Pdgfr β, supplied by St Johns Laboratory, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Boster Bio pdgfrb antibodies
Figure 4: Analyses of fusion gene <t>CD53-PDGFRB.</t> (A) RT-PCR analysis of CD53, PDGFRB and CD53-PDGFRB fusion transcripts in cell lines DERL-2 and DERL-7. NTC: no template control. (B) RQ-PCR analysis of CD53 in selected cell lines and primary cells. (C) RQ-PCR analysis of PDGFRB in selected cell lines and primary cells (left). Western blot analysis of PDGFRB (middle) and phospho- PDGFRB (right) was performed in selected cell lines. TUBA served as loading control. (D) RQ-PCR analysis of PDGFD in selected cell lines and primary cells (left). Data for PDGFD protein levels in the supernatants as obtained by ELISA are indicated (right). (E) RQ-PCR analysis for PDGFRB <t>and</t> <t>MSX1</t> of DERL-2 cells (left) and DERL-7 cells (right) treated for siRNA-mediated knockdown of PDGFRB. (F) RQ-PCR analysis of MSX1 in DERL-2 cells and DERL-7 cells treated with 1 ng/ml and 10 ng/ml recombinant PDGFD (left). RQ- PCR analysis for PDGFD and MSX1 of DERL-7 cells treated for siRNA-mediated knockdown of PDGFD (middle). RQ-PCR analysis for MSX1 of DERL-2 and DERL-7 cells treated with PDGFR kinase-inhibitor Dasatinib (right). (G) RQ-PCR analysis for PDGFRB and AUTS2 of DERL-2 cells (above) and DERL-7 cells (below) treated for siRNA-mediated knockdown of PDGFRB.
Pdgfrb Antibodies, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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OriGene mouse anti pdgfrb
Figure 4: Analyses of fusion gene <t>CD53-PDGFRB.</t> (A) RT-PCR analysis of CD53, PDGFRB and CD53-PDGFRB fusion transcripts in cell lines DERL-2 and DERL-7. NTC: no template control. (B) RQ-PCR analysis of CD53 in selected cell lines and primary cells. (C) RQ-PCR analysis of PDGFRB in selected cell lines and primary cells (left). Western blot analysis of PDGFRB (middle) and phospho- PDGFRB (right) was performed in selected cell lines. TUBA served as loading control. (D) RQ-PCR analysis of PDGFD in selected cell lines and primary cells (left). Data for PDGFD protein levels in the supernatants as obtained by ELISA are indicated (right). (E) RQ-PCR analysis for PDGFRB <t>and</t> <t>MSX1</t> of DERL-2 cells (left) and DERL-7 cells (right) treated for siRNA-mediated knockdown of PDGFRB. (F) RQ-PCR analysis of MSX1 in DERL-2 cells and DERL-7 cells treated with 1 ng/ml and 10 ng/ml recombinant PDGFD (left). RQ- PCR analysis for PDGFD and MSX1 of DERL-7 cells treated for siRNA-mediated knockdown of PDGFD (middle). RQ-PCR analysis for MSX1 of DERL-2 and DERL-7 cells treated with PDGFR kinase-inhibitor Dasatinib (right). (G) RQ-PCR analysis for PDGFRB and AUTS2 of DERL-2 cells (above) and DERL-7 cells (below) treated for siRNA-mediated knockdown of PDGFRB.
Mouse Anti Pdgfrb, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Boster Bio pdgfr β antibodies
Figure 4. Immunolocalization of (A-C) platelet-derived growth factor-B (PDGF-B) and (D-F) PDGF receptor-β <t>(PDGFR-β)</t> in brain tissue of (A,D) controls, (B,E) lipopolysaccharide (LPS) group at 72 h, and the (C,F) glutamine (Gln) treatment group at 72 h. PDGF-B immunoreactivity was observed in the cyto plasm, PDGFR-β immunoreactivity was observed in the membrane of cortical neurons.
Pdgfr β Antibodies, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Flow cytometry antibody panel used for characterization.

Journal: Biomedical Reports

Article Title: Comparative study of biological characteristics of mesenchymal stem cells isolated from mouse bone marrow and peripheral blood

doi: 10.3892/br.2019.1236

Figure Lengend Snippet: Flow cytometry antibody panel used for characterization.

Article Snippet: Supplier CD29 PE HMs1-1 130-102-994 Miltenyi Biotec, Inc. CD44 FITC IM7.8.1 130-102-511 Miltenyi Biotec, Inc. CD105 PE MJ7/18 130-102-548 Miltenyi Biotec, Inc. CD90.2 FITC 30-H12 130-120-091 Miltenyi Biotec, Inc. CD146 FITC ME-9F1 130-102-230 Miltenyi Biotec, Inc. SCa-1 PE D7 130-102-832 Miltenyi Biotec, Inc. CD45 FITC 30F11.1 130-110-658 Miltenyi Biotec, Inc. CD140b PE APB5 130-118-457 Miltenyi Biotec, Inc. Open in a separate window CD, cluster of differentiation; PE, phycoethrin; FITC, fluorescein isothiocyanate.

Techniques: Flow Cytometry

Flow cytometric analysis of BM-MSCs and PB-MSCs. BM-MSCs were positive CD29 and negative for other markers, while PB-MSCs were positive for CD146, CD29, and CD140b and negative for Sca-1, CD44, CD45, CD90 and CD105. CD, cluster of differentiation; BM-MSCs, bone marrow-mesenchymal stromal cells; PB, peripheral blood; FITC, fluorescein isothiocyanate; PE, phycoerythrin.

Journal: Biomedical Reports

Article Title: Comparative study of biological characteristics of mesenchymal stem cells isolated from mouse bone marrow and peripheral blood

doi: 10.3892/br.2019.1236

Figure Lengend Snippet: Flow cytometric analysis of BM-MSCs and PB-MSCs. BM-MSCs were positive CD29 and negative for other markers, while PB-MSCs were positive for CD146, CD29, and CD140b and negative for Sca-1, CD44, CD45, CD90 and CD105. CD, cluster of differentiation; BM-MSCs, bone marrow-mesenchymal stromal cells; PB, peripheral blood; FITC, fluorescein isothiocyanate; PE, phycoerythrin.

Article Snippet: Supplier CD29 PE HMs1-1 130-102-994 Miltenyi Biotec, Inc. CD44 FITC IM7.8.1 130-102-511 Miltenyi Biotec, Inc. CD105 PE MJ7/18 130-102-548 Miltenyi Biotec, Inc. CD90.2 FITC 30-H12 130-120-091 Miltenyi Biotec, Inc. CD146 FITC ME-9F1 130-102-230 Miltenyi Biotec, Inc. SCa-1 PE D7 130-102-832 Miltenyi Biotec, Inc. CD45 FITC 30F11.1 130-110-658 Miltenyi Biotec, Inc. CD140b PE APB5 130-118-457 Miltenyi Biotec, Inc. Open in a separate window CD, cluster of differentiation; PE, phycoethrin; FITC, fluorescein isothiocyanate.

Techniques:

Figure 4: Analyses of fusion gene CD53-PDGFRB. (A) RT-PCR analysis of CD53, PDGFRB and CD53-PDGFRB fusion transcripts in cell lines DERL-2 and DERL-7. NTC: no template control. (B) RQ-PCR analysis of CD53 in selected cell lines and primary cells. (C) RQ-PCR analysis of PDGFRB in selected cell lines and primary cells (left). Western blot analysis of PDGFRB (middle) and phospho- PDGFRB (right) was performed in selected cell lines. TUBA served as loading control. (D) RQ-PCR analysis of PDGFD in selected cell lines and primary cells (left). Data for PDGFD protein levels in the supernatants as obtained by ELISA are indicated (right). (E) RQ-PCR analysis for PDGFRB and MSX1 of DERL-2 cells (left) and DERL-7 cells (right) treated for siRNA-mediated knockdown of PDGFRB. (F) RQ-PCR analysis of MSX1 in DERL-2 cells and DERL-7 cells treated with 1 ng/ml and 10 ng/ml recombinant PDGFD (left). RQ- PCR analysis for PDGFD and MSX1 of DERL-7 cells treated for siRNA-mediated knockdown of PDGFD (middle). RQ-PCR analysis for MSX1 of DERL-2 and DERL-7 cells treated with PDGFR kinase-inhibitor Dasatinib (right). (G) RQ-PCR analysis for PDGFRB and AUTS2 of DERL-2 cells (above) and DERL-7 cells (below) treated for siRNA-mediated knockdown of PDGFRB.

Journal: Oncotarget

Article Title: Deregulated expression of NKL homeobox genes in T-cell lymphomas.

doi: 10.18632/oncotarget.26929

Figure Lengend Snippet: Figure 4: Analyses of fusion gene CD53-PDGFRB. (A) RT-PCR analysis of CD53, PDGFRB and CD53-PDGFRB fusion transcripts in cell lines DERL-2 and DERL-7. NTC: no template control. (B) RQ-PCR analysis of CD53 in selected cell lines and primary cells. (C) RQ-PCR analysis of PDGFRB in selected cell lines and primary cells (left). Western blot analysis of PDGFRB (middle) and phospho- PDGFRB (right) was performed in selected cell lines. TUBA served as loading control. (D) RQ-PCR analysis of PDGFD in selected cell lines and primary cells (left). Data for PDGFD protein levels in the supernatants as obtained by ELISA are indicated (right). (E) RQ-PCR analysis for PDGFRB and MSX1 of DERL-2 cells (left) and DERL-7 cells (right) treated for siRNA-mediated knockdown of PDGFRB. (F) RQ-PCR analysis of MSX1 in DERL-2 cells and DERL-7 cells treated with 1 ng/ml and 10 ng/ml recombinant PDGFD (left). RQ- PCR analysis for PDGFD and MSX1 of DERL-7 cells treated for siRNA-mediated knockdown of PDGFD (middle). RQ-PCR analysis for MSX1 of DERL-2 and DERL-7 cells treated with PDGFR kinase-inhibitor Dasatinib (right). (G) RQ-PCR analysis for PDGFRB and AUTS2 of DERL-2 cells (above) and DERL-7 cells (below) treated for siRNA-mediated knockdown of PDGFRB.

Article Snippet: The following antibodies were used: MSX1 (R & D Systems), alpha-Tubulin (Sigma), PDGFRB (R & D Systems), phospho-PDGFRB (Aviva Systems Biology, Eching, Germany), NKX2-2 (Aviva Systems Biology) and PITX1 (Abnova, Taipei, Taiwan).

Techniques: Reverse Transcription Polymerase Chain Reaction, Control, Western Blot, Enzyme-linked Immunosorbent Assay, Knockdown, Recombinant

Figure 6: Analysis of BMP4. (A) RQ-PCR analysis of BMP4 in selected cell lines and primary cells. Data for BMP4 protein levels in the supernatants as obtained by ELISA are indicated (insert). (B) RQ-PCR analysis of MSX1 in DERL-2 and DERL-7 cells treated with recombinant BMP4 (left) and with dorsomorphin (DM) (right). (C) RQ-PCR analysis for PDGFRB and BMP4 of DERL-2 cells (left) and DERL-7 cells (right) treated for siRNA-mediated knockdown of PDGFRB. (D) RQ-PCR analysis of BMP4 in DERL-2 (left) and DERL-7 (right) treated with 1 ng/ml or 10 ng/ml recombinant PDGFD. (E) RQ-PCR analysis for PDGFD and BMP4 of DERL-7 cells treated for siRNA-mediated knockdown of PDGFD.

Journal: Oncotarget

Article Title: Deregulated expression of NKL homeobox genes in T-cell lymphomas.

doi: 10.18632/oncotarget.26929

Figure Lengend Snippet: Figure 6: Analysis of BMP4. (A) RQ-PCR analysis of BMP4 in selected cell lines and primary cells. Data for BMP4 protein levels in the supernatants as obtained by ELISA are indicated (insert). (B) RQ-PCR analysis of MSX1 in DERL-2 and DERL-7 cells treated with recombinant BMP4 (left) and with dorsomorphin (DM) (right). (C) RQ-PCR analysis for PDGFRB and BMP4 of DERL-2 cells (left) and DERL-7 cells (right) treated for siRNA-mediated knockdown of PDGFRB. (D) RQ-PCR analysis of BMP4 in DERL-2 (left) and DERL-7 (right) treated with 1 ng/ml or 10 ng/ml recombinant PDGFD. (E) RQ-PCR analysis for PDGFD and BMP4 of DERL-7 cells treated for siRNA-mediated knockdown of PDGFD.

Article Snippet: The following antibodies were used: MSX1 (R & D Systems), alpha-Tubulin (Sigma), PDGFRB (R & D Systems), phospho-PDGFRB (Aviva Systems Biology, Eching, Germany), NKX2-2 (Aviva Systems Biology) and PITX1 (Abnova, Taipei, Taiwan).

Techniques: Enzyme-linked Immunosorbent Assay, Recombinant, Knockdown

Figure 7: Analysis of NKX2-2. (A) RQ-PCR analysis of NKX2-2 in primary cells and selected cell lines. Western blot analysis of NKX2-2 in selected cell lines (insert). TUBA served as loading control. (B) RQ-PCR analysis for NKX2-2 and MSX1 of DERL-2 cells (left) and DERL-7 cells (right) treated for siRNA-mediated knockdown of NKX2-2. (C) Reporter gene assay was performed in NIH-3T3 cells using a promoter fragment of MSX1. Forced expression of NKX2-2 resulted in increased reporter gene activity. (D) RQ-PCR analysis for MSX1 and NKX2-2 of DERL-7 cells treated for siRNA-mediated knockdown of NKX2-2. (E) RQ-PCR analysis for PDGFRB and NKX2-2 of DERL-2 cells (left) and DERL-7 cells (right) treated for siRNA-mediated knockdown of PDGFRB.

Journal: Oncotarget

Article Title: Deregulated expression of NKL homeobox genes in T-cell lymphomas.

doi: 10.18632/oncotarget.26929

Figure Lengend Snippet: Figure 7: Analysis of NKX2-2. (A) RQ-PCR analysis of NKX2-2 in primary cells and selected cell lines. Western blot analysis of NKX2-2 in selected cell lines (insert). TUBA served as loading control. (B) RQ-PCR analysis for NKX2-2 and MSX1 of DERL-2 cells (left) and DERL-7 cells (right) treated for siRNA-mediated knockdown of NKX2-2. (C) Reporter gene assay was performed in NIH-3T3 cells using a promoter fragment of MSX1. Forced expression of NKX2-2 resulted in increased reporter gene activity. (D) RQ-PCR analysis for MSX1 and NKX2-2 of DERL-7 cells treated for siRNA-mediated knockdown of NKX2-2. (E) RQ-PCR analysis for PDGFRB and NKX2-2 of DERL-2 cells (left) and DERL-7 cells (right) treated for siRNA-mediated knockdown of PDGFRB.

Article Snippet: The following antibodies were used: MSX1 (R & D Systems), alpha-Tubulin (Sigma), PDGFRB (R & D Systems), phospho-PDGFRB (Aviva Systems Biology, Eching, Germany), NKX2-2 (Aviva Systems Biology) and PITX1 (Abnova, Taipei, Taiwan).

Techniques: Western Blot, Control, Knockdown, Reporter Gene Assay, Expressing, Activity Assay

Figure 8: Analysis of PITX1 and SETD2. (A) RQ-PCR analysis of PITX1 in selected cell lines and primary cells (left). Western blot analysis of PITX1 in selected cell lines (right). TUBA served as loading control. (B) RQ-PCR analysis for PITX1 and MSX1 of DERL-2 (left) and DERL-7 (right) treated for siRNA-mediated knockdown of PITX1. (C) RQ-PCR analysis for PDGFRB and PITX1 of DERL-2 (left) and DERL-7 (right) treated for siRNA-mediated knockdown of PDGFRB. (D) RQ-PCR analysis of SETD2 in selected cell lines and primary cells. (E) RQ-PCR analysis for SETD2 and PITX1 of DERL-2 (left) and DERL-7 (right) treated for siRNA-mediated knockdown of SETD2. (F) RQ-PCR analysis of PITX1 in DERL-7 cells treated with indicated amounts of recombinant IL2. (G) RQ-PCR analysis for SETD2 and PITX1 of LOUCY cells treated for siRNA-mediated knockdown of SETD2.

Journal: Oncotarget

Article Title: Deregulated expression of NKL homeobox genes in T-cell lymphomas.

doi: 10.18632/oncotarget.26929

Figure Lengend Snippet: Figure 8: Analysis of PITX1 and SETD2. (A) RQ-PCR analysis of PITX1 in selected cell lines and primary cells (left). Western blot analysis of PITX1 in selected cell lines (right). TUBA served as loading control. (B) RQ-PCR analysis for PITX1 and MSX1 of DERL-2 (left) and DERL-7 (right) treated for siRNA-mediated knockdown of PITX1. (C) RQ-PCR analysis for PDGFRB and PITX1 of DERL-2 (left) and DERL-7 (right) treated for siRNA-mediated knockdown of PDGFRB. (D) RQ-PCR analysis of SETD2 in selected cell lines and primary cells. (E) RQ-PCR analysis for SETD2 and PITX1 of DERL-2 (left) and DERL-7 (right) treated for siRNA-mediated knockdown of SETD2. (F) RQ-PCR analysis of PITX1 in DERL-7 cells treated with indicated amounts of recombinant IL2. (G) RQ-PCR analysis for SETD2 and PITX1 of LOUCY cells treated for siRNA-mediated knockdown of SETD2.

Article Snippet: The following antibodies were used: MSX1 (R & D Systems), alpha-Tubulin (Sigma), PDGFRB (R & D Systems), phospho-PDGFRB (Aviva Systems Biology, Eching, Germany), NKX2-2 (Aviva Systems Biology) and PITX1 (Abnova, Taipei, Taiwan).

Techniques: Western Blot, Control, Knockdown, Recombinant

Figure 4. Immunolocalization of (A-C) platelet-derived growth factor-B (PDGF-B) and (D-F) PDGF receptor-β (PDGFR-β) in brain tissue of (A,D) controls, (B,E) lipopolysaccharide (LPS) group at 72 h, and the (C,F) glutamine (Gln) treatment group at 72 h. PDGF-B immunoreactivity was observed in the cyto plasm, PDGFR-β immunoreactivity was observed in the membrane of cortical neurons.

Journal: Molecular medicine reports

Article Title: Protective effects of glutamine in a rat model of endotoxemia.

doi: 10.3892/mmr.2012.1007

Figure Lengend Snippet: Figure 4. Immunolocalization of (A-C) platelet-derived growth factor-B (PDGF-B) and (D-F) PDGF receptor-β (PDGFR-β) in brain tissue of (A,D) controls, (B,E) lipopolysaccharide (LPS) group at 72 h, and the (C,F) glutamine (Gln) treatment group at 72 h. PDGF-B immunoreactivity was observed in the cyto plasm, PDGFR-β immunoreactivity was observed in the membrane of cortical neurons.

Article Snippet: Rabbit anti-rat NF-κB, PDGF-B, and PDGFR-β antibodies and ABC kits were purchased from Boster (Wuhan, China).

Techniques: Derivative Assay, Membrane

Figure 6. Western blot analysis of PDGF receptor-β (PDGFR-β) protein at various time points following injection of lipopolysaccharide (LPS). Upper row depicts the control group from left to right as 1-5 (2, 6, 12, 24 and 72 h) and the treatment group as 6-10 (2, 6, 12, 24 and 72 h). Lower row depicts the control group from left to right as 1-5 (2, 6, 12, 24 and 72 h) and the LPS group as 6-10 (2, 6, 12, 24 and 72 h).

Journal: Molecular medicine reports

Article Title: Protective effects of glutamine in a rat model of endotoxemia.

doi: 10.3892/mmr.2012.1007

Figure Lengend Snippet: Figure 6. Western blot analysis of PDGF receptor-β (PDGFR-β) protein at various time points following injection of lipopolysaccharide (LPS). Upper row depicts the control group from left to right as 1-5 (2, 6, 12, 24 and 72 h) and the treatment group as 6-10 (2, 6, 12, 24 and 72 h). Lower row depicts the control group from left to right as 1-5 (2, 6, 12, 24 and 72 h) and the LPS group as 6-10 (2, 6, 12, 24 and 72 h).

Article Snippet: Rabbit anti-rat NF-κB, PDGF-B, and PDGFR-β antibodies and ABC kits were purchased from Boster (Wuhan, China).

Techniques: Western Blot, Injection, Control